study on cryo-preservation of theileria annulata schizont infected cell line vaccine strain s15
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abstract
the purpose of this study was to examine the potential application of sorbitol, sucrose and gelatin in combination with glycerol in order to theileria annulata infected cell line cryo-preservation. the efficiency of the freezing methods was compared by assessing the viability and plating efficiency of cells after resuscitation, dilution and equilibration in cell culture medium. as a result, the conventional cryo-preservative medium containing 5% peg treated bovine serum and 10% glycerol successfully cryo-preserved cell as efficiently as the standard medium containing 80% fbs and 10% glycerol and was superior to all thirteen different experimental cryo-preservative media. a crucial result was obtained among the thirteen different serum-free media which showed that the 0.2m sorbitol+10% glycerol method yielded cells with viability of 54% and good plating efficiency results. it was concluded that the conventional method would be the best and ideal for the cryo-preservation and storage of theileria annulata-infected cells for vaccine purposes to date.
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Journal title:
archives of razi institutePublisher: razi vaccine & serum research institute (rvsri)
ISSN 0365-3439
volume 67
issue 2 2016
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